Reconstructions of refractive index tomograms via discrete algebraic reconstruction technique

摘要

Optical diffraction tomography (ODT) provides three-dimensional refractiveindex (RI) tomograms of a transparent microscopic object. However, because ofthe finite numerical aperture of objective lenses, ODT has the limited accessto diffracted light and suffers from poor spatial resolution, particularlyalong the axial direction. To overcome the limitation of the quality of RItomography, we present an algorithm that accurately reconstructs RI tomographyusing preliminary information that the RI values of a specimen are discrete anduniform. Through simulations and experiments on various samples, includingmicrospheres, red blood cells, and water droplets, we show that the proposedmethod can precisely reconstruct RI tomograms of samples with discrete anduniform RI values in the presence of the missing information and noise.